Cetyltrimethylammonium bromide (CTAB), a type of cationic detergent, is used to release and complex total cellular nucleic acids. CTAB forms an insoluble bond with nucleic acids when the initial NaCl concentration is reduced to ~0.5 M. Through the supernatant, polysaccharides, phenolic compounds and other enzyme-inhibitory contaminants found in plant cells are effectively removed, as most of them do not precipitate under these conditions.
CTAB
CTAB is a useful compound for forming insoluble complexes with cellular nucleic acids and removing plant contaminants, which allows for better purification of DNA particles.
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