SMOChem ™ deoxynucleotide mixture (dNTP) is an aqueous solution containing an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5.
Use instead of dTTP in PCR and RT-PCR protocols to avoid carryover of previous amplifications. Substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also known as UDG) can be added to a PCR premix to remove uracil from any contaminating PCR product, thus avoiding false positives. Each batch of dUTP is tested to ensure specific DNA amplification and absence of nuclease activity.