SMOChem ™ deoxynucleotide mixture (dNTP) is an aqueous solution containing an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5.

Ultrapure dATP solution supplied as sodium salt in purified water (pH 8.5).

Ultrapure dCTP solution supplied as sodium salt in purified water (pH 8.5).

Ultrapure dGTP solution supplied as sodium salt in purified water (pH 8.5).

Ultrapure dTTP solution supplied as sodium salt in purified water (pH 8.5).

Use instead of dTTP in PCR and RT-PCR protocols to avoid carryover of previous amplifications. Substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also known as UDG) can be added to a PCR premix to remove uracil from any contaminating PCR product, thus avoiding false positives. Each batch of dUTP is tested to ensure specific DNA amplification and absence of nuclease activity.