The kit is designed for rapid isolation of genomic DNA from animal tissue such as mouse tail, ear clips, hair or saliva, human, fish and insect tissues. There is no need for the use of phenol or overnight digestion, the lysate can be used directly as a PCR template. The one-tube procedure minimizes the possibility of cross-contamination between samples. The whole procedure takes less than 15 minutes and this kit is an example of speed and efficiency. The kit is suitable for high PCR screening of large-scale samples. This kit also works for other applications, such as genotyping and sequencing.