Real-time polymerase chain reaction (qPCR) works by allowing amplification of a fragment of interest from the total DNA of a particular microorganism or pathogen. Initially, a DNA sample is taken as a starting point and then successively amplified in a PCR reaction. In the case of the PATHOGEN Fish line kits, amplification is detected by the use of probes (Taqman) that report such amplification. These probes are based on the technology of an emitting fluorophore and a repressor dye or quencher.
The kit for the molecular detection (qPCR) of
Streptococcus iniae
molecular detection kit (qPCR), which comes in a 100-reaction package, includes as a positive control bacterial genomic DNA from the strain of
Streptococcus iniae
ATCC® 29177TM (Pier and Madin) from the American Type Culture Collection (ATCC) bio-bank.
Kit components
| Component | Quantity (Cat. No. PF002) |
| Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A | 1 vial (1.3 ml) |
| Solution B+ | 1 vial (100 ul) |
| Solution C‡ | 1 vial (100 ul) |
| Nuclease Free Water (NTC) | 1 vial (1.2 mll) |
* Master Mix, +Pathogen Mix, ‡ Positive control.
