The master mix contains all the components for PCR except for the templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR master mix is supplied as a ready-to-use 5X / 2X concentrated mixture of Taq DNA polymerase, reaction buffer, MgCl 2 (TP1120 contains MgSO 4), dNTP and enzyme stabilizer that enables efficient PCR template amplification and allows the user to prepare their PCR reactions conveniently. This product is supplied with a 6X DNA loading dye (blue) containing two follow up dyes (xylene cyanol FF and bromophenol blue) for post PCR analysis using agarose gel electrophoresis.
Features
-DNA polymerase 5 ‘→ 3′ activity.
-No detectable 3’→ 5′ exonuclease activity (proofreading)
-Generates PCR products with 3’-dA
High-performance PCR
-High reproducibility
-Reduced pipetting errors
Applications
-Routine PCR
-PCR of colonies
High-performance PCR
-DNA fragments amplification up to 8 kb
-Generation of PCR products for AT cloning.
-DNA labeling

Storage

-4 ° C for 6 months
-20 ° C for 24 months