The DNA Ladder DM5100 is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easy reference. In addition, three tracking dyes, xylene cyanol FF, bromophenol blue and orange G that mimic 4,000 bp, 500 bp and 50 bp dsDNA migration during electrophoresis are added for real-time monitoring.
Features
– Sharp bands
– Quick reference: improved belts
– Ready to use: pre-mixed with loading colorant for direct loading
– Stable : storage at room temperature for 6 months
Source
PCR products extracted with phenol and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Source
PCR products extracted with phenol and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 25,000 bp
Concentration
88.7 µg / 500 µl
Recommended load volume
5 µl / well
Storage
-Ambient temperature for 6 months
4 ° C for 12 months
-20 ° C for 36 months