Real-time quantitative reverse transcription PCR (qRT-PCR or RT-qPCR) is a powerful tool for the analysis of gene expression and detection of viruses whose genetic material is ribonucleotidic in nature. Brilliant II QRT-PCR, AffinityScript Two-Step Master Mix provides an optimized system for one-step QRT-PCR applications. A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source.
The MasterMix V2 qPCR kit is used in combination with PathoSEEK® and/or FemINIDCAtor® detection panels. To use a new qPCR-based assay that is uncontaminated and provides an internal plant DNA control for each reaction. It is a simple two-step protocol, flexible and compatible with automation and multiple real-time detection platforms. Our qPCR kit includes the following items:
Reaction buffer (10x)
Decontamination enzyme (10 units / uL)
qPCR Master mix (5x)
Nuclease-free water
The NEW qPCR Master Kit version 3 has been optimized to work with the new 5-color Aspergillus assays. It is compatible with all our other presence/absence tests in the PathoSEEK® menu. The Version 3 kit should NOT be used with our total count tests.
Our qPCR kit includes the following items:
Reaction buffer (10x)
Decontamination enzyme (10 units / uL)
It is a kit for synthesizing high quality first strand cDNA.
RNA, respectively.
SMOChem ™ deoxynucleotide mixture (dNTP) is an aqueous solution containing an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5.
Ultrapure dATP solution supplied as sodium salt in purified water (pH 8.5).
Ultrapure dCTP solution supplied as sodium salt in purified water (pH 8.5).
Ultrapure dGTP solution supplied as sodium salt in purified water (pH 8.5).
Ultrapure dTTP solution supplied as sodium salt in purified water (pH 8.5).
Use instead of dTTP in PCR and RT-PCR protocols to avoid carryover of previous amplifications. Substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also known as UDG) can be added to a PCR premix to remove uracil from any contaminating PCR product, thus avoiding false positives. Each batch of dUTP is tested to ensure specific DNA amplification and absence of nuclease activity.
ExcelTaq ™ Taq DNA polymerase is a recombinant
Thermostable Taq DNA polymerase expressed and
purified from an E. colistrain carrying the cloned gene.
With a high DNA synthesis rate and high thermostability, ExcelTaq ™ Taq DNA polymerase is suitable for
for common and specialized PCR applications.
ExcelTaq ™ Taq DNA polymerase is a recombinant
Thermostable Taq DNA polymerase expressed and
purified from an E. colistrain carrying the cloned gene.
With a high DNA synthesis rate and high thermostability, ExcelTaq ™ Taq DNA polymerase is suitable for
for common and specialized PCR applications.