The PIK3CA mutation activates the PI3K (phosphatidylinositol 3-kinase) protein and PI3K activates several receptor tyrosine kinase signaling pathways. Activated tyrosine kinase represses the function and excitometabolism of the protein that inhibits cell survival and division. These abnormal changes initiate the cancer development process by activating the protein involved in cell growth. Point mutations in PIK3CA are detected with approx. 80% frequency in exon 9 (interpreted as helical part of the PI3K protein) and exon 20 (interpreted as part of the kinase), while other types of mutations are also observed in many different locations. PIK3CA mutations are found with a frequency of 25-40% in various types of cancer such as colorectal cancer, gastric cancer, lung cancer, brain cancer, endometrial cancer, ovarian cancer, breast cancer and more. The PNAClamp ™ PIK3CA mutation detection kit utilizes modified PCR technology, using optimized PNA probes that bind tightly to wild-type DNA templates. This tight binding to wild-type DNA templates does not result in amplification of the wild-type DNA template in the PCR reaction, whereas mutated genes, especially with SNPs, PCR amplification is processed for multiplication of the mutated DNA sequences.