The fidelity of G-HiFi ™ DNA polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of DNA polymerase G-HiFi ™ is achieved by mixing the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ buffer includes special ingredients that suppress non-specific amplification as well as the plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ buffer, G-HiFi™ DNA polymerase is able to amplify most templates (up to 40 kb of lambda DNA) containing GC-rich sequences.
Features
-DNA polymerase 5 ‘→ 3’ activity.

-Exonuclease activity (proofreading) 3 ‘→ 5’

-High reaction speed: 7 seconds / kb

-High fidelity: 70 times higher than Taq polymerase.

-Generates blunt-ended amplicons

-High elongation capacity (up to 40 kb)

-Thermostable for more than 10 hours at 95 ° C

Storage

-20 ° C for 24 months