With a sensitive fluorescent dye of high specific affinity towards double-stranded DNA (dsDNA), the FluoroDye ™ fluorescent DNA loading dye has negligible background fluorescence and makes the decolorization process unnecessary. The FluoroDye™ fluorescent DNA loading dye allows the user to immediately visualize the electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye ™ DNA fluorescent loading dye is compatible with both the conventional UV gel illumination system and the less damaging long wavelength blue light illumination system. The emission of FluoroDye ™ bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Characteristics:

-Excellent for premixes with DNA samples

-Sensitivity: 0.14 ng (DNA)

-A safer alternative to EtBr

-Compatibility: suitable for blue or ultraviolet light

-Increased cloning efficiency (blue light)

Composition

FluoroDye ™ DNA fluorescent loading dye is stored at 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing bromophenol blue, xylene cyanol FF and orange G as follow-up dyes.

Storage

-Protected from direct light
-20 ° C for 24 months