RNA-dependent DNA polymerase capable of generating the first cDNA strand using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. ExcelRT ™ reverse transcriptase is capable of routinely synthesizing first-strand cDNA. > 8 kb in a temperature range between 37 ~ 50 ° C. Features -High yield-Thermostable, up to 50 ° C, during first-strand synthesis-High processivity, generating cDNA up to 8 kb-Ribonuclease activity of RNase H reduced-No detectable exonucleolytic 3’→ 5′-Ribonuclease correction function-Thermally stable for at least 4 weeks when stored at 4 ° C Application -Generation of first-strand cDNA from total RNA or mRNA.Suitable for generating cDNA from RNA with a strong secondary structure that can be reduced at a temperature up to 50°C. Storage -20 ° C for 24 months.
ExcelRT™ Reverse Transcriptase, (200 U/μl, 20000 U) – RP1000
SKU
Pcto-0057
Categories Amplification, cDNA synthesis, Products