The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for real-time reverse transcription and quantitative analysis of a specific target RNA using a one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of a one-step RT enzyme mix and a 2X one-step qPCR master mix, is a conveniently designed kit for cDNA synthesis and highly specific qPCR in a single tube. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) contains a thermostable ExcelRT™ reverse transcriptase and an RNase inhibitor RNAok™. Consequently, the One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA over a wide temperature range, from 42 to 60 °C, and is active against RNase A, RNase B and RNase C. Containing a specialized hot-start Taq DNA polymerase, which greatly reduces primer dimer formation and can be activated within 2 minutes, the 2X One-Step qPCR Master Mix exhibits high specificity and is suitable for a rapid cycling program. This master mix includes the ROX reference dye for normalization of each RT-qPCR assay.

Features

High performance

Reverse transcription over a wide temperature range (42-60°C)

High specificity

Suitable for a fast cycle program

Includes ROX reference dye

Storage and recommendations for use

– Aliquoting is recommended to avoid multiple freeze-thaw cycles (stable up to 30 freeze-thaw cycles).

– Protect from light

-20°C for 12 months