Real-time quantitative reverse transcription PCR (qRT-PCR or RT-qPCR) is a powerful tool for the analysis of gene expression and detection of viruses whose genetic material is ribonucleotidic in nature. Brilliant II QRT-PCR, AffinityScript Two-Step Master Mix provides an optimized system for one-step QRT-PCR applications. A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source.
The MasterMix V2 qPCR kit is used in combination with PathoSEEK® and/or FemINIDCAtor® detection panels. To use a new qPCR-based assay that is uncontaminated and provides an internal plant DNA control for each reaction. It is a simple two-step protocol, flexible and compatible with automation and multiple real-time detection platforms. Our qPCR kit includes the following items:
Reaction buffer (10x)
Decontamination enzyme (10 units / uL)
qPCR Master mix (5x)
Nuclease-free water
The NEW qPCR Master Kit version 3 has been optimized to work with the new 5-color Aspergillus assays. It is compatible with all our other presence/absence tests in the PathoSEEK® menu. The Version 3 kit should NOT be used with our total count tests.
Our qPCR kit includes the following items:
Reaction buffer (10x)
Decontamination enzyme (10 units / uL)