ExcelTaq ™ 2X Q-PCR Master Mix (TaqMan, ROX)
is a ready-to-use reagent with all essential elements
components for real-time quantitative PCR (qPCR)
except primers, TaqMan probes and templates. the
The masterbatch includes a 5′ to 3′ exonuclease activity for
cleaving TaqMan probes that hybridize to the target
sequence, releasing the fluorophore during probe
displacement. With the TaqMan probes, the master mix
has high specificity and high sensitivity (Fig. 1).