This product is supplied as a 5X concentrated mix containing all essential PCR ingredients except for templates and primers. In addition, the mixture contains an electrophoresis tracking dye (bromophenol blue) and a safer fluorescent DNA staining dye, which allows the user to track the electrophoresis process in real time and eliminates the need for the staining process. The resulting PCR reaction mixture is dense enough to load directly onto a gel with TAE or TBE for electrophoresis.

Features

-DNA polymerase 5 ‘→ 3’ activity.

-No detectable 3 ‘→ 5′ exonuclease activity (proofreading)<

-Generates PCR products with 3’-dA overhangs

High-performance PCR

-High reproducibility

-Reduced pipetting errors

<-For direct loading in electrophoresis analysis

-DNA bands can be visualized directly under ultraviolet light or 470 nm blue light illumination.

Applications

-Routine PCR

-PCR of colonies

High-performance PCR

-DNA fragments amplification up to 8 kb

-Generation of PCR products for AT cloning.

-DNA labeling

Storage

-Protected from light and avoids multiple freeze/thaw cycles

-4 ° C for 6 months

-20 ° C for 24 months