The master mix includes a 5′ to 3′ exonuclease activity to cleave TaqMan probes that hybridize to the target sequences, releasing the fluorophore(s) during polymerase displacement on the probe. With TaqMan probes, the master mix has high specificity and high sensitivity. The ExcelTaq ™ 2X Q-PCR Master Mix (TaqMan, ROX) contains Taq HotStart in an optimized buffer that enables sensitive and accurate amplification, real-time monitoring of the amplification process and simultaneous quantification of specific DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. The ExcelTaq ™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready to use and greatly reduces pipetting errors while greatly improving process reproducibility.

Features
-High specificity
-High sensitivity
-With ROX reference dye

Storage
-Protect from light.
-Aliquot to avoid multiple freeze-thaw cycles.
-20 ° C for 12 months