The ExcelRT\u2122 One-Step RT-qPCR kit (TaqMan, ROX) is designed for real-time reverse transcription and quantitative analysis of a specific target RNA using a one-step reaction. The ExcelRT\u2122 One-Step RT-qPCR kit (TaqMan, ROX), consisting of a one-step RT enzyme mix and a 2X one-step qPCR master mix, is a conveniently designed kit for cDNA synthesis and highly specific qPCR in a single tube. The ExcelRT\u2122 One-Step RT-qPCR kit (TaqMan, ROX) contains a thermostable ExcelRT\u2122 reverse transcriptase and an RNase inhibitor RNAok\u2122. Consequently, the One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA over a wide temperature range, from 42 to 60 \u00b0C, and is active against RNase A, RNase B and RNase C. Containing a specialized hot-start Taq DNA polymerase, which greatly reduces primer dimer formation and can be activated within 2 minutes, the 2X One-Step qPCR Master Mix exhibits high specificity and is suitable for a rapid cycling program. This master mix includes the ROX reference dye for normalization of each RT-qPCR assay.<\/p>\n
Features<\/p>\n
High performance<\/p>\n
Reverse transcription over a wide temperature range (42-60\u00b0C)<\/p>\n
High specificity <\/p>\n
Suitable for a fast cycle program<\/p>\n
Includes ROX reference dye<\/p>\n
Storage and recommendations for use<\/p>\n
– Aliquoting is recommended to avoid multiple freeze-thaw cycles (stable up to 30 freeze-thaw cycles).<\/p>\n
– Protect from light<\/p>\n
-20\u00b0C for 12 months<\/p>\n","protected":false},"excerpt":{"rendered":"
The ExcelRT One-Step RT-qPCR kit (TaqMan, ROX) is designed for real-time reverse transcription and quantitative analysis of a specific target RNA using a one-step reaction.<\/p>\n","protected":false},"featured_media":6002,"template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_joinchat":[]},"product_brand":[],"product_cat":[154,168,151],"product_tag":[],"class_list":{"0":"post-9786","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-amplification","7":"product_cat-onestep-rt-pcr-and-rt-qpcr","8":"product_cat-products","9":"pa_marca-smobio","10":"desktop-align-center","11":"tablet-align-left","12":"mobile-align-left","14":"first","15":"instock","16":"shipping-taxable","17":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product\/9786","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/types\/product"}],"version-history":[{"count":0,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product\/9786\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/media\/6002"}],"wp:attachment":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/media?parent=9786"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_brand?post=9786"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_cat?post=9786"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_tag?post=9786"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}