This product is supplied as a 5X concentrated mix containing all essential PCR ingredients except for templates and primers. In addition, the mixture contains an electrophoresis tracking dye (bromophenol blue) and a safer fluorescent DNA staining dye, which allows the user to track the electrophoresis process in real time and eliminates the need for the staining process. The resulting PCR reaction mixture is dense enough to load directly onto a gel with TAE or TBE for electrophoresis.<\/p>\n
Features<\/p>\n
-DNA polymerase 5 ‘\u2192 3’ activity.<\/p>\n
-No detectable 3 ‘\u2192 5′ exonuclease activity (proofreading)<<\/p>\n
-Generates PCR products with 3’-dA overhangs<\/p>\n
High-performance PCR<\/p>\n
-High reproducibility<\/p>\n
-Reduced pipetting errors<\/p>\n
<-For direct loading in electrophoresis analysis<\/p>\n
-DNA bands can be visualized directly under ultraviolet light or 470 nm blue light illumination.<\/p>\n
Applications<\/p>\n
-Routine PCR<\/p>\n
-PCR of colonies<\/p>\n
High-performance PCR<\/p>\n
-DNA fragments amplification up to 8 kb<\/p>\n
-Generation of PCR products for AT cloning.<\/p>\n
-DNA labeling<\/p>\n
Storage<\/p>\n
-Protected from light and avoids multiple freeze\/thaw cycles<\/p>\n
-4 \u00b0 C for 6 months<\/p>\n
-20 \u00b0 C for 24 months<\/p>\n","protected":false},"excerpt":{"rendered":"
ExcelTaq \u2122 5X Fluorescent PCR Master Mix is a ready-to-use mix for amplifying specific DNA fragments. It is designed to serve as a master mix for virtually all PCR applications.<\/p>\n","protected":false},"featured_media":6134,"template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_joinchat":[]},"product_brand":[],"product_cat":[154,156,151,157],"product_tag":[],"class_list":{"0":"post-9618","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-amplification","7":"product_cat-pcr-en","8":"product_cat-products","9":"product_cat-reagents","10":"pa_marca-smobio","11":"desktop-align-center","12":"tablet-align-left","13":"mobile-align-left","15":"first","16":"instock","17":"shipping-taxable","18":"product-type-simple"},"acf":[],"_links":{"self":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product\/9618","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/types\/product"}],"version-history":[{"count":0,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product\/9618\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/media\/6134"}],"wp:attachment":[{"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/media?parent=9618"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_brand?post=9618"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_cat?post=9618"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/gentechbio.com\/en\/wp-json\/wp\/v2\/product_tag?post=9618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}