{"id":9491,"date":"2023-05-17T16:49:18","date_gmt":"2023-05-17T21:49:18","guid":{"rendered":"https:\/\/gentechbio.com\/producto\/molecular-diagnostic-kit-qpcr-for-detection-of-streptococcus-iniae-pf0002\/"},"modified":"2023-07-30T23:32:10","modified_gmt":"2023-07-31T04:32:10","slug":"molecular-diagnostic-kit-qpcr-for-detection-of-streptococcus-iniae-pf0002","status":"publish","type":"product","link":"https:\/\/gentechbio.com\/en\/producto\/molecular-diagnostic-kit-qpcr-for-detection-of-streptococcus-iniae-pf0002\/","title":{"rendered":"Molecular diagnostic kit (qPCR) for detection of Streptococcus iniae PF0002"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Real-time polymerase chain reaction (qPCR) works by allowing amplification of a fragment of interest from the total DNA of a particular microorganism or pathogen. Initially, a DNA sample is taken as a starting point and then successively amplified in a PCR reaction. In the case of the PATHOGEN Fish line kits, amplification is detected by the use of probes (Taqman) that report such amplification. These probes are based on the technology of an emitting fluorophore and a repressor dye or quencher.  <\/span><\/p>\n<p><span style=\"font-weight: 400;\">The kit for the molecular detection (qPCR) of <\/span><i><br \/>\n  <span style=\"font-weight: 400;\">Streptococcus iniae<\/span><br \/>\n<\/i><span style=\"font-weight: 400;\">molecular detection kit (qPCR), which comes in a 100-reaction package, includes as a positive control bacterial genomic DNA from the strain of <\/span><i><br \/>\n  <span style=\"font-weight: 400;\">Streptococcus iniae<\/span><br \/>\n<\/i><span style=\"font-weight: 400;\"> ATCC<\/span><span style=\"font-weight: 400;\">\u00ae<\/span><span style=\"font-weight: 400;\"> 29177<\/span><span style=\"font-weight: 400;\">TM<\/span><span style=\"font-weight: 400;\"> (Pier and Madin) from the American Type Culture Collection (ATCC) bio-bank.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Kit components<\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td><span style=\"font-weight: 400;\">Component<\/span><\/td>\n<td><span style=\"font-weight: 400;\">Quantity (Cat. No. PF002)<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 400;\">Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A* Solution A<\/span><\/td>\n<td><span style=\"font-weight: 400;\">1 vial (1.3 ml)  <\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 400;\">Solution B<\/span><b>+<\/b><\/td>\n<td><span style=\"font-weight: 400;\">1 vial (100 ul)<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 400;\">Solution C<\/span><b>\u2021<\/b><\/td>\n<td><span style=\"font-weight: 400;\">1 vial (100 ul)<\/span><\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-weight: 400;\">Nuclease Free Water (NTC)<\/span><\/td>\n<td><span style=\"font-weight: 400;\">1 vial (1.2 mll)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><b>*<\/b> <span style=\"font-weight: 400;\">Master Mix, <\/span><b>+<\/b><span style=\"font-weight: 400;\">Pathogen Mix, <\/span><b>\u2021<\/b> <span style=\"font-weight: 400;\">Positive control.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p><span style=\"font-weight: 400;\">Kit for molecular detection <\/span><span style=\"font-weight: 400;\">(qPCR) <\/span><span style=\"font-weight: 400;\">of <\/span><i><br \/>\n  <span style=\"font-weight: 400;\">Streptococcus iniae<\/span><br \/>\n<\/i><span style=\"font-weight: 400;\"> with Taqman probe system<\/span><\/p>\n","protected":false},"featured_media":11356,"template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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